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Identification of Teriparatide by Sodium dodecyl sulfate polyacrylamide (SDS-PAGE)

Kashish Chaudhary2, Priyanka Chaudhary1, Anu Prakash1, Anubhuti Goyal1, Muthusamy Kalaivani1*, Meenakshi Dahiya1, Vivekanandan Kalaiselvan1

1 Biologics Division, Indian Pharmacopoeia Commission, Sector-23, Raj Nagar, Ghaziabad (U.P), India.

2 Amity Institute of Biotechnology, Amity University, Sector-125, Gautam Budh Nagar (U.P), India.

Corresponding Author

Dr. Muthusamy Kalaivani
Principal Scientific Officer,
Indian Pharmacopoeia Commission,
Ministry of Health and Family Welfare, Govt. of India,
Sector-23, Raj Nagar, Ghaziabad-201002, India.

Running Title

Identification of Teriparatide by SDS-PAGE

Abstract

Background: SDS-PAGE is a widely used technique for identifying and characterizing proteins based on their size and charge. Teriparatide, a synthetic form of the N-terminal fragment of human parathyroid hormone (PTH), can be identified through SDS-PAGE by its distinct molecular weight (~4.2 kDa) and migration pattern. This method enables the verification of its purity and confirmation of its protein structure during quality control processes.

Aim: The aim of this study is to identify teriparatide through SDS-PAGE by evaluating its molecular weight and migration characteristics in comparison to a reference standard. This technique allows for the accurate determination of its purity and helps confirm the integrity of the recombinant protein. Additionally, it provides a reliable method for distinguishing teriparatide from other protein contaminants. Ultimately, this approach supports quality control and ensures the consistency of the therapeutic agent.

Material & Method: In this study, we evaluated teriparatide via SDS-PAGE, checking its molecular weight for similarity and conducting qualitative analysis in comparison to a reference standard.

Results: Teriparatide's molecular weight and purity were evaluated using SDS-PAGE. Teriparatide (~4.2 kDa) is represented by a single band on polyacrylamide gel that was stained with silver nitrate. No additional bands were observed, suggesting minimal degradation or the presence of impurities. These results confirm the identity and purity of the teriparatide sample.

Conclusion: SDS-PAGE successfully identified teriparatide by revealing its distinct molecular weight and migration pattern. The technique confirmed the protein's purity, ensuring that it met the required standards for therapeutic use. The results also validated the structural integrity of the recombinant protein, distinguishing it from potential contaminants. SDS-PAGE proved to be an effective and reliable method for quality control in the production of teriparatide. Overall, this approach guarantees the consistency and safety of the therapeutic agent for clinical application.

Keywords

Teriparatide, SDS-PAGE, human parathyroid hormone, electrophoresis

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Publication Details

  • Article ID: O0701031 RA Preprint
  • Received: 10/07/2025
  • Accepted: 30/08/2025
  • Published: 30/08/2025
  • DOI: Pending

Citations

Chaudhary, K., Chaudhary, P., Prakash, A., Goyal, A., Kalaivani, M., Dahiya, M., & Kalaiselvan, V. (2025). Identification of Teriparatide by Sodium dodecyl sulfate polyacrylamide (SDS-PAGE). Journal of Allbiosolution, 7(1), 311–316.

Kashish Chaudhary et al. Identification of Teriparatide by Sodium dodecyl sulfate polyacrylamide (SDS-PAGE). Journal of Allbiosolution, 2025, 7(1): 311-316.

K. Chaudhary et al. Identification of Teriparatide by Sodium dodecyl sulfate polyacrylamide (SDS-PAGE). Allbiosolution, 2025, 7(1): 311-316.