Disadvantages of iPS technology

Girish K. Srivastava

1 IOBA, Universidad de Valladolid, Valladolid, Spain.


Induced pluripotent stem (iPS) cells are a type of stem cell generated by reprogramming adult cells, typically skin cells, through the introduction of specific genes called "reprogramming factors." Among the necessary factors, a combination of four transcription factors (TF) known as OSKM is commonly used. OSKM stands for four-octamer binding protein 34 (OCT3/4), sex-determining region Y-box 2 (SOX2), Kruppel-like factor 4 (KLF4) and cellular myelocytomatosis (MYC). These factors, when introduced into host cells, initiate the reprogramming process. Researchers have proposed more than 30 different combinations of these transcription factors for reprogramming, but only a few combinations have been proven effective for reprogramming somatic cells in both humans and mice. This underscores the complexity and specificity of the reprogramming process, as not all combinations yield successful results. Nevertheless, this reprogramming process allows the cells to revert to a pluripotent state. The factors are usually introduced using nonintegrative or integrative non viral or viral vectors, resetting the gene expression patterns of the adult cells and effectively transforming them into pluripotent stem cells. Pluripotent cells, similar to embryonic stem cells, have the ability to differentiate into various cell types in the body, such as neurons, heart cells, liver cells, and more. Nevertheless, there are some notable drawbacks of iPS technology.

Keywords: iPSC, iPS technology device


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Article ID: A0401020 RA Preprint 
Received: 01/12/2022 
Accepted: 07/12/2022
Published: 31/12/2022

GK Srivastava. Disadvantages of iPS technology. Journal of Allbiosolution, 2022, 4(1):201-203
GK Srivastava. Disadvantages of iPS technology. Allbiosolution, 2022, 4(1):201-203