YIGSR sequence contained elastin like recombinamer for sustaining retinal pigment epithelium as a possible tool for retinal regenerative medicine

Girish K. Srivastava1, 3, Laura Martin 2, David Rodríguez-Crespo1, Amar K. Singh 1, Manuel J. Gayoso 1, José C. Rodríguez-Cabello 2, José C. Pastor1,3

1.     Instituto Universitario de Oftalmobiología Aplicada, Universidad de Valladolid, 47011 Valladolid, Spain.
2.     BIOFORGE Group, Universidad de Valladolid, CIBER-BBN, 47011 Valladolid, Spain.
3.     Centro en Red de Medicina Regenerativa y Terapia Celular de Castilla y León, 47011 Valladolid, Spain.          


Retinal pigment epithelial (RPE) cell replacement therapy is promising for AMD patients. However, it needs a suitable surface to sustain RPE characteristics. Constructed elastin like recombinamer containing YIGSR sequence (ELR-YIGSR), using solvent-casting onto glass cover slips followed by cross-linking, was evaluated along with TCP polystyrene (control) for ARPE19 cells sustainability (cell adhesion, viability, proliferation, epithelial characteristics and cellular junctions) at 4, 24, 72 and 120 hours. Cell viability was over 90% at 72 and 120 hours. Numbers of cells adhered (42.29±24.24%) at 4 hours and grew at 4, 24, 72, and 120 hours on ELR-YIGSR surface was significantly lower (25±16, 63.5±25, 420±60 and 662±45) than that of control TCP polystyrene (49.5±25.21, 190.5±57.27, 768±67.81 and 810.3±145.2). Microscopic results confirmed epithelial characteristics (hexagonal morphology, Ecad and ZO-1protein expressions, and tight and gap junctions). Thus, it confirmed ELR-YIGSR surface sustained RPE cell characteristics. However, this needs subsequent studies with fresh human RPE cells to conclude its application in transplantation.

Keywords: ELR; YIGSR; RPE; AMD; Biomaterial


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Ms. Ref. No.: A0101005     Reviewer 1   Reviewer 2
Received: 20042018
Accepted: 30062018
Article in Press: 01072018 
Published Online: 15072018

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